<p>Position Summary</p> <p>The Alt Lab is seeking a highly motivated and detail-oriented individual for a Research Assistant III role. Our Alt lab investigates the molecular mechanisms underlying V(D)J recombination, class switch recombination, and somatic hypermutation in developing and activated B lymphocytes. We apply these insights to generate mouse models that support HIV-1 research, vaccine development, and antibody-based therapies. This role combines hands-on experimental work with technical expertise, regulatory compliance, and leadership responsibilities, including management of lab operations, mentoring junior staff, and maintaining adherence to institutional protocols.</p> <p>Key Responsibilities</p> <ul> <li>Perform advanced embryo manipulation techniques, including microinjection of pre-implantation embryos with mouse stem cells and aseptic surgical transfer to the uterus or oviduct. </li><li>Generation and maintenance of transgenic and knockout mouse models in a barrier-level facility. </li><li>Maintenance of mouse colonies, including specialized care for immunodeficient models. </li><li>Troubleshoots procedures if experimental results are unexpected or quality of specimens is poor. </li><li>Independently decides which techniques may be appropriate for specific experimental investigations and carries out those experiments with minimal supervision or independently. </li><li>Critically evaluates experimental results independently and provides summaries or interpretation of results to PI. </li><li>Coaches RA I and RA II, interns, Co Ops on basic lab techniques. </li><li>Calculates, graphs and compiles data obtained, maintaining records and logs of work performed; uses computer to perform statistical analyses. </li></ul> <p>Minimum Qualifications</p> <p>Education:</p> <ul> <li>Bachelor's Degree in STEM or Psychology; Master's preferred </li></ul> <p>Experience:</p> <ul> <li>Bachelor's degree and 3 years of relevant experience OR Master's and 1 year of relevant experience </li><li>Experience with mouse embryonic stem cell culture and derivation is a plus </li></ul> <p>The posted pay range is Boston Children's reasonable and good-faith expectation for this pay at the time of posting.</p> <p>Any base pay offer provided depends on skills, experience, education, certifications, and a variety of other job-related factors. Base pay is one part of a comprehensive benefits package that includes flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.</p>