<p>Department: MED-Neuroscience</p> <p>Salary/Grade: NEX/11</p> <p>Target hiring range for this position will be between $19.89-$24.86 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.</p> <p>Job Summary:</p> <p>Responsibilities include stereotaxic surgeries (viral delivery and implantations), coordination of longitudinal neural and behavioral data collection, and maintenance of transgenic mouse colonies. The technologist will also contribute to histology, data processing, and integration of neural, behavioral, and circuit-mapping datasets, while helping to implement rigorous, reproducible workflows and open-science data sharing practices aligned with the goals of the collaborative network.</p> <p>Specific Responsibilities:</p> <p>Molecular Biology:</p> <ul> <li>DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting </li><li>PCR, RT-PCR </li><li>Plasmid preparation </li><li>Cloning, mapping and sequencing of DNA </li><li>Recombinant virus production and purification </li></ul> <p>Cellular Biology:</p> <ul> <li>Cell culture, cell isolation </li><li>Functional studies </li><li>Transfection </li></ul> <p>Histology:</p> <ul> <li>Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining </li><li>Immunohistochemistry </li></ul> <p>Immunology:</p> <ul> <li>Flow cytometry </li><li>ELISA, Elispot assay </li><li>Cytokine assay </li><li>Antibody production and purification </li></ul> <p>Animal:</p> <ul> <li>Husbandry </li><li>Breeding and screening transgenic mice </li><li>Dissections </li><li>Blood collection </li><li>Surgical procedures </li><li>Database management of colony </li></ul> <p>Administration</p> <ul> <li>Maintains detailed records of results which may include collecting, extracting & entering data. </li><li>Manipulates, interprets &/or presents results. </li><li>Prepares charts & graphs for presentations & publications. </li><li>Performs scientific literature searches in support of research. </li><li>Completes portions of grant applications, manuscripts & publications. </li></ul> <p>Finance</p> <ul> <li>Tracks & summarizes lab supply expenses. </li><li>Verifies accuracy of invoices. </li><li>Orders &/or maintains inventory of reagent, mice &/or lab supplies. </li></ul> <p>Supervises</p> <ul> <li>Trains students. </li></ul> <p>Miscellaneous</p> <p>Performs other duties as assigned.</p> <p>Minimum Qualifications:</p> <ul> <li>Successful completion of a full 4-year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience. </li></ul> <p>Minimum Competencies: (Skills, knowledge, and abilities.)</p> <ul> <li>Demonstrated laboratory experience; </li><li>Demonstrated ability to follow detailed instructions as required by research protocols; </li><li>Demonstrated computer skills, including spreadsheets and basic computations; </li><li>Attention to detail and accurate record keeping; </li><li>Ability to work independently and in a team environment; </li><li>Manual dexterity. </li></ul> <p>Benefits:</p> <p>At Northwestern, we are proud to provide meaningful and competitive benefits. The available benefits package for this position will include health, dental, vision, disability, and life insurance; paid vacation and holidays; paid medical/sick and parental leave; tuition benefits for the employee and dependents; pre-tax and flex spending accounts for commuting and dependent care; generous retirement savings options; and wellness programs. For a comprehensive overview of available benefits, including eligibility details, visit us at https://www.northwestern.edu/hr/benefits/index.html to learn more.</p> <p>Work-Life and Wellness:</p> <p>Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities, and adopt and maintain healthy lifestyles.</p> <p>We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at https://www.northwestern.edu/hr/benefits/work-life/index.html to learn more.</p> <p>Professional Growth & Development:</p> <p>Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at https://www.northwestern.edu/hr/learning/index.html to learn more.</p> <p>Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. View Northwestern's non-discrimination statement. Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process.</p> <p>#LI-GY2</p>