<p>Essential Duties</p> <ol> <li>Designs, plans, implements and evaluates complex multifaceted research projects/systems, including method development, adaptation and validation. 2. Collaborates with PI to define research endeavors and the development of research hypothesis and approach. 3. Solves complex methodology, protocol, procedural and research problems through design of techniques, procedures and policies that will achieve research goals. 4. Develops long range plans for supplies and equipment to ensure smooth operation of laboratory. 5. Investigates, analyzes and evaluates complex data, data collection systems and methods to reach scientific conclusions and ensures the integrity of research data. 6. Collaborates with PI to coordinate major lab renovations working with Facilities, Health and Safety, and Purchasing to ensure deadlines, safety standards and purchases are according to plans and within budgets. 7. Prepares scientific reports and papers for research proposals and published reports. 8. May perform other duties as assigned. </li></ol> <p>Required Education and Experience</p> <p>Master's Degree in a scientific discipline and three years of experience or an equivalent combination of education and experience.</p> <p>Background Check Requirements</p> <p>All candidates for employment will be subject to pre-employment background screening for this position, which may include motor vehicle, DOT certification, drug testing and credit checks based on the position description and job requirements. All offers are contingent upon the successful completion of the background check. For additional information on the background check requirements and process visit "Learn about background checks" under the Applicant Support Resources section of Careers on the It's Your Yale website.</p> <p>Position Focus:</p> <p>Under the direction of the two Principal investigators and working in collaboration with the laboratory manager this position will support an intra lab core designed to meet the needs of laboratory researchers in 10x, 16s, Spatial Transcriptomics, and whole genome sequencing and related bioinformatics analysis. Using knowledge from computer science to manipulate and process complex research data. Data management involves capturing information pertaining to biological and cellular activities based on DNA research. Duties and tasks are complex in nature where considerable judgment, independent action and a high degree of initiative are required in resolving problems and making recommendations. Work is performed with a high level of autonomy with demonstrated ability to independently determine methods and procedures on new assignments.</p> <p>Principal Responsibilities:</p> <p>Spatial Transcriptomics- CosMx Imaging, to quantify gene expression (mRNA transcripts) within their spatial context in fixed tissue at the cellular level. Meet with investigators to design experiments. Train lab members, slide preparation, run samples, store, retrieve, and analyze data.</p> <p>Whole genome sequencing- determine the complete DNA sequence to include all coding and non-coding regions. Meet with investigators to design experiments. Train lab members, slide preparation, run samples, store, retrieve, and analyze data.</p> <p>10X sequencing-gem preparation, processing, and analysis: for differential gene expression, pathway analysis, trajectories, and ligand interactions.</p> <p>16s sequencing-preparation, processing, and bacterial composition analysis.</p> <p>Build and utilize programs using computational formulas to determine outcomes based for Immunobiology research.</p> <p>Develop software, creating query routines, and building relational databases.</p> <p>Possess advanced knowledge of algorithms used to solve problems based on biological information and data using multiple different calculations.</p> <p>10X gem preparation, processing, and analysis: for differential gene expression, pathway analysis, trajectories, and ligand interactions.</p> <p>16s sequencing preparation, processing, and bacterial composition analysis.</p> <p>Oversite of a centralized laboratory inventory of all sequencing reagents to maximize utilization and minimize waste.</p> <p>Maintenance of laboratory owned sequencers.</p> <p>Interact with related vendors.</p> <p>Stay current with new technology in the field.</p> <p>Prepare scientific reports and figures for research in progress meeting, proposals and publication.</p> <p>Preferred Education, Experience and Skills:</p> <p>Proven ability to translate and normalize gene expression data into heat maps and libraries. Advanced knowledge of mathematics and molecular biology. Previous experience in the bioinformatic based research. Strong writing and research skills.</p> <p>Posting Disclaimer</p> <p>The intent of this job description is to provide a representative summary of the essential functions that will be required of the position and should not be construed as a declaration of specific duties and responsibilities of the particular position. Employees will be assigned specific job-related duties through their hiring departments.</p>